Fig. 5.

Wnt/β-catenin activation upregulates Lama2 expression in PCs, but not in astrocytes and ECs. (A-L) Primary mouse brain PCs, astrocytes and ECs treated with either DMSO or CHIR99021 (CHIR), followed by staining for Lef1, DAPI and either Pdgfrβ, Pdgfrα or lectin (A,E,I), or Lama2, DAPI and either NG2, GFAP or lectin (C,G,K). Yellow arrowheads indicate Lef1⁺ nuclei. B,F,J show percentage of cells with nuclear Lef1 expression. H,J,L show quantification of Lama2 expression by corresponding cell types. (M-R) Untreated or recombinant Wnt3a-treated primary mouse brain PCs, astrocytes and ECs stained for Lama2, DAPI and then NG2 (M), GFAP (O) or lectin (Q). N,P,R show the quantification of Lama2 expression by various cell types. n=3 or 4 independent experiments; Data are mean±s.e.m., analyzed with two-tailed unpaired Student's t-test: NS, not significant, **P<0.02.