Figure 4. Naringin (Nar) prevented acetaminophen (APAP)-induced mitochondrial injury in HepG2 cells. A, Detection of changes in the TMRE/MitoTracker Green fluorescence signal by confocal microscopy (scale bar 20 μm). B, Flow chart of high-content analysis monitoring of MMP changes. C and D, TMRE intensity indicating the MMP at 0 and 3 h in different groups (scale bar 100 μm). E, Changes in the glutamate dehydrogenase (GDH) level, an index of mitochondrial injury, in different groups after 24 h APAP-induced injury. F, Red/green fluorescence ratio in JC-1-stained cells. Data are reported as means±SD (n=3). *P<0.05, **P<0.01, and ***P<0.001 vs the APAP group (ANOVA).
