Table 1. Integration sites of mCherry-E3L in horizontal gene transfer (HGT) viruses and importance of disrupted genes for virus replication.

Isolate #	Integration site	Essential vs. nonessential
HGT1	Intergenic between H4L and H5R	Presumably nonessential
HGT2	I8R	Essential (Gross and Shuman, 1996)
HGT3	H4L	Essential (Kane and Shuman, 1992)
HGT4	G1L	Essential (Hedengren-Olcott et al., 2004)
HGT5	I4L	Nonessential (Child et al., 1990; Gammon et al., 2010)
HGT6	D1R	Essential (Hassett et al., 1997)
HGT7	A40R	Nonessential (Wilcock et al., 1999)
HGT8	A9L	Essential (Yeh et al., 2000)
HGT9 and HGT10	D9R	Nonessential (Parrish and Moss, 2006)
HGT11	B4R	Nonessential (Burles et al., 2014)
HGT12	Intergenic between A49R and A50R	Presumably nonessential
HGT13	F13L	Nonessential (Blasco and Moss, 1991)
HGT14	H4L	Essential (Kane and Shuman, 1992)
HGT15	C19L/B25R	Nonessential (Perkus et al., 1991)
HGT16	A26L	Nonessential (Howard et al., 2008; Chang et al., 2019)
HGT17	G6R	Nonessential (Senkevich et al., 2008)
HGT18	D6R	Essential (Broyles and Fesler, 1990)
HGT19	D11L	Essential (Seto et al., 1987)
HGT20	Intergenic between N2L and M1L	Presumably nonessential