| Genetic reagent (M. musculus) |
Sptlc1fl/fl mice |
Alexaki et al., 2017 (PMID:28100772) |
RRID:MMRRC_042294-UCD
|
|
| Genetic reagent (M. musculus) |
Cdh5-Cre-ERT2 mice |
Sörensen et al., 2009 (PMID:19144989) |
RRID:MGI:3848984
|
|
| Genetic reagent (M. musculus) |
Sphk1fl/fl mice |
Pappu et al., 2007 (PMID:17363629) |
RRID:MMRRC_030038-UCD
|
|
| Genetic reagent (M. musculus) |
Sphk2-/- mice |
Mizugishi et al., 2005 (PMID:16314531) |
RRID:IMSR_JAX:019140
|
|
| Genetic reagent (M. musculus) |
Apom-/- mice |
Christoffersen et al., 2008 (PMID:18006500) |
RRID:MGI:3772574
|
|
| Cell line (Human) |
Human eubmilical vein endothelial cells |
Lonza |
C2519A |
|
| Antibody |
Anti-CD31 MicroBeads (rat monoclonal) |
Miltenyi Biotec |
130-097-418; RRID:AB_2814657
|
|
| Antibody |
Anti-CD45 MicroBeads (rat monoclonal) |
Miltenyi Biotec |
130-052-301; RRID:AB_2877061
|
|
| Antibody |
(PE)-conjugated Anti-CD31 (rat monoclonal) |
Biolegend |
102508; RRID:AB_312915
|
For EC Isolation (1:250) |
| Antibody |
(APC)-conjugated Anti-mouse CD45 (rat monoclonal) |
Biolegend |
103112; RRID:AB_312977
|
For EC Isolation (1:250) |
| Antibody |
Anti-SPTLC1 (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-374143; RRID:AB_10917035
|
For Western Biotting (1:1000) |
| Antibody |
Anti-phospho eNOS (rabbit monoclonal) |
Cell Signaling Techonology |
9571; RRID:AB_329837
|
For Western Biotting (1:1000) |
| Antibody |
Anti-eNOS (rabbit polyclonal) |
BD Biosciences |
Cat #:610296; RRID:AB_397691
|
For Western Biotting (1:1000) |
| Antibody |
Anti-β-Actin (mouse monoclonal) |
Sigma-Aldrich |
Cat# A5316; RRID:AB_476743
|
For Western Biotting (1:5000) |
| Antibody |
Anti-rabbit IgG-HRP conjugated (goat polyclonal) |
Abcam |
Cat# ab205718; RRID:AB_2819160
|
For Western Biotting (1:5000) |
| Antibody |
Anti-mouse IgG-HRP conjugated |
Millipore |
AP-124P; RRID:AB_9045
|
For Western Biotting (1:5000) |
| Antibody |
Anti-ERK (rabbit polyclonal) |
Cell Signaling Techonology |
Cat# 9102; RRID:AB_330744
|
For Western Blotting (1:2000) |
| Antibody |
Anti-phospho ERK (rabbit monoclonal) |
Cell Signaling Techonology |
Cat# 9106; RRID:AB_331768
|
For Western Blotting (1:2000) |
| Antibody |
Anti-ApoM (rabbit monoclonal) |
Abcam |
ab91656; RRID:AB_204916
|
For Western Blotting (1:2000) |
| Antibody |
Anti-NG2 (rabbit polyclonal) |
Sigma-Aldrich |
AB5320; RRID: AB_91789
|
For Immunofluorescence (1:200) |
| Antibody |
Anti Phospho-histone H3 (Ser28, HTA28) (rat monoclonal) |
Biolegend |
641001; RRID: AB_1227660
|
For Immunofluorescence (1:200) |
| Antibody |
Anti-ESM1 (goat polyclonal) |
R&D Systems |
AF1999; RRID: AB_2101810
|
For Immunofluorescence (1:200) |
| Antibody |
Anti-ERG (rabbit monoclonal) |
Abcam |
Cat# ab92513; RRID:AB_2630401
|
For Immunofluorescence (1:500) |
| Antibody |
Alexa Fluor 488-conjugated Anti- ERG (rabbit monoclonal) |
Abcam |
ab196374; RRID:AB_2889273
|
For Immunofluorescence (1:200) |
| Antibody |
Alexa Fluor 647-conjugated Anti- ERG (rabbit monoclonal) |
Abcam |
ab196149 |
For Immunofluorescence (1:200) |
| Antibody |
Anti-LEF1 (rabbit monoclonal) |
Cell Signaling Techonology |
2230; RRID:AB_823558
|
For Immunofluorescence (1:200) |
| Antibody |
Anti-TFRC (rat monoclonal) |
Novus Biologicals |
NB100-64979; RRID: AB_962622
|
For Immunofluorescence (1:200) |
| Antibody |
Anti-CLDN5 (rabbit polyclonal) |
Invitrogen |
34–1600; RRID: AB_2533157
|
For Immunofluorescence (1:200) |
| Antibody |
Anti-MFSD2A (rabbit polyclonal) |
Gift from David Silver Lab |
PMID: 23209793 |
For Immunofluorescence (1:200) |
| Antibody |
Anti-Actin, α-Smooth Muscle - Cy3 Antibody (mouse monoclonal) |
Sigma-Aldrich |
C6198; RRID:AB_476856
|
For Immunofluorescence (1:200) |
| Antibody |
Human VE-Cadherin Antibody (goat polyclonal) |
R&D Systems |
AF938; RRID:AB_355726
|
For Immunofluorescence (1:200) |
| Chemical compound or drug |
Tamoxifen |
Sigma-Aldrich |
T5648 |
|
| Chemical compound or drug |
Corn Oil |
Sigma-Aldrich |
C8267 |
|
| Chemical compound or drug |
Liberase TM Research Grade |
Sigma-Aldrich |
5401127001 |
|
| Chemical compound or drug |
ACK lysis buffer |
Thermo Fisher Scientific |
A1049201 |
|
| Chemical compound or drug |
Alexa Fluor 647-Conjugated Isolectin GS-IB4 From Griffonia Simplicifolia |
Thermo Fisher Scientific |
I32450 |
For Immunofluorescence (1:500) |
| Chemical compound or drug |
cOmplete Protease Inhibitor Cocktail |
Roche |
11836145001 |
|
| Chemical compound or drug |
Pierce ECL Western Blotting Substrate |
Thermo Fisher Scientific |
32106 |
|
| Chemical compound or drug |
Bovine Serum Albumin lyophilized powder, essentially fatty acid free |
Sigma-Aldrich |
A6003 |
|
| Chemical compound or drug |
Fluoromount-G slide mounting medium |
SouthernBiotech |
0100–01 |
|
| Chemical compound or drug |
Sphingosine-1-Phosphate (d18:1) |
Avanti Lipids |
860492 P |
|
| Chemical compound or drug |
sphingosine-1-phosphate-d7 |
Avanti Lipids |
860659 P-1mg |
|
| Chemical compound or drug |
Sphingosine (d18:1) |
Avanti Lipids |
860490 P |
|
| Chemical compound or drug |
Sphinganine (d18:0) |
Avanti Lipids |
860498 P |
|
| Chemical compound or drug |
Recombinant Mouse VEGF-164 (carrier-free) |
Biolegend |
583102 |
|
| Chemical compound or drug |
Acetaminophen |
Sigma-Aldrich |
A7085 |
|
| Chemical compound or drug |
Myriocin |
Cayman Chemical |
63150 |
|
| Chemical compound or drug |
C16 Ceramide (d18:1/16:0) |
Cayman Chemical |
10681 |
|
| Commercial assay or kit |
DC Protein Assay Reagents |
Bio-Rad |
5000116 |
|
| Commercial assay or kit |
AST Activity Assay Kit |
Sigma-Aldrich |
MAK055 |
|
| Commercial assay or kit |
Glutathione Assay Kit |
Cayman Chemical |
703002 |
|
| Commercial assay or kit |
Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit |
Thermo Fisher Scientific |
V34403 |
|
| Software, algorithm |
Fiji |
NIH |
RRID:SCR_002285
|
|
| Software, algorithm |
Graphpad Prism 9.0 |
Graphpad Software |
RRID:SCR_002798
|
|
| Software, algorithm |
Affinity Photo |
Serif (Europe) |
RRID:SCR_016951
|
|
| Software, algorithm |
Skyline |
MacCoss Lab Software |
RRID: SCR_014080
|
|
| Sequence-based reagent |
Sptlc1Flox For |
Alexaki et al., 2017 (PMID:28100772) |
RRID:MMRRC_042294-UCD
|
For genotyping Sptlc1 Flox allele 5’-GGG TTC TAT GGC ACA TTT GGT AAG-3’
|
| Sequence-based reagent |
Sptlc1Flox Rev |
Alexaki et al., 2017 (PMID:28100772) |
RRID:MMRRC_042294-UCD
|
For genotyping Sptlc1 Flox allele 5’-CTG TTA CTT CTT GCC AGT GGA C-3’
|
| Sequence-based reagent |
Sptlc1 Flox Deletion For |
Alexaki et al., 2017 (PMID:28100772) |
RRID:MMRRC_042294-UCD
|
For genotyping Sptlc1 Flox Deletion allele 5’-CAG AGC TAA TGG AAA GGT GTC-3’
|
| Sequence-based reagent |
Sptlc1 Flox Deletion Rev |
Alexaki et al., 2017 (PMID:28100772) |
RRID:MMRRC_042294-UCD
|
For genotyping Sptlc1 Flox Deletion allele 5’-CTG TTA CTT CTT GCC AGT GGA C-3’
|
| Sequence-based reagent |
Cdh5-Cre For |
Sörensen et al., 2009(PMID:19144989) |
RRID:MGI:3848984
|
For genotyping Cdh5-Cre allele5’-TCC TGA TGG TGC CTA TCC TC-3
|
| Sequence-based reagent |
Cdh5-Cre Rev |
Sörensen et al., 2009 (PMID:19144989) |
RRID:MGI:3848984
|
For genotyping Cdh5-Cre allele 5’-CCT GTT TTG CAC GTT CAC CG-3’
|
| Sequence-based reagent |
Sphk1 Flox Common For |
Pappu et al., 2007 (PMID:17363629) |
RRID:MMRRC_030038-UCD
|
For genotyping Sphk1 Flox allele 5-GGA CCT GGC TAT GGA ACC-3'
|
| Sequence-based reagent |
Sphk1 Flox Rev |
Pappu et al., 2007 (PMID:17363629) |
RRID:MMRRC_030038-UCD
|
For genotyping Sphk1 Flox allele 5-ATG TTT CTT TCG AGT GAC CC-3'
|
| Sequence-based reagent |
Sphk1Flox Rev Deletion |
Pappu et al., 2007 (PMID:17363629) |
RRID:MMRRC_030038-UCD
|
For genotyping Sphk1 Flox Deletion allele 5-AAT GCC TAC TGC TTA CAA TAC-3'
|
| Sequence-based reagent |
Sphk2 Mutant For |
Mizugishi et al., 2005 (PMID:16314531) |
RRID:IMSR_JAX:019140
|
For genotyping Sphk2 allele 5'-CTC GTG CTT TAC GGT ATC GC-3'
|
| Sequence-based reagent |
Sphk2 Common Rev |
Mizugishi et al., 2005 (PMID:16314531) |
RRID:IMSR_JAX:019140
|
For genotyping Sphk2 allele 5'-CAC TGC ACC CAG TGT GAA TC-3'
|
| Sequence-based reagent |
Sphk2 Mutant Rev |
Mizugishi et al., 2005 (PMID:16314531) |
RRID:IMSR_JAX:019140
|
For genotyping Sphk2 allele 5'-TCA TCC TGC TGC CCC TTA C-3'
|
| Sequence-based reagent |
Apom For 1 |
Christoffersen et al., 2008 (PMID:18006500) |
RRID:MGI:3772574
|
For genotyping Apom allele 5'-CAC CCA GCA ACT CAT CCT TT-3'
|
| Sequence-based reagent |
Apom For 2 |
Christoffersen et al., 2008 (PMID:18006500) |
RRID:MGI:3772574
|
For genotyping Apom allele 5'-GCA GCG CAT CGC CTT CTA TC-3'
|
| Sequence-based reagent |
Apom Rev |
Christoffersen et al., 2008 (PMID:18006500) |
RRID:MGI:3772574
|
For genotyping Apom allele 5'-TCT TCC CCA CAC CCT AGC TC-3'
|
