TABLE 1.
PTP levels of CVID PBMCs stimulated with anti-human IgM antibodya
Patient | Densitometry units
|
||
---|---|---|---|
P | N | P/N ratio | |
P1 | 110,983 | 161,778 | 0.68 |
P2 | 294,174 | 643,100 | 0.45 |
P3 | 362,861 | 372,472 | 0.97 |
P5 | 198,263 | 300,583 | 0.65 |
P6 | 265,215 | 226,370 | 1.17 |
P7 | 204,904 | 196,402 | 1.04 |
P8 | 727,849 | 1,102,152 | 0.66 |
P10 | 334,680 | 594,231 | 0.56 |
P11 | 413,190 | 465,654 | 0.88 |
P12 | 331,587 | 465,654 | 0.71 |
P13 | 433,695 | 683,251 | 0.63 |
The results represent the sum of densitometric units of all the bands in each lane. Each row represents the results of one experiment carried out with the cells of one CVID patient (P) and one normal donor (N) after stimulation for 10 min with anti-human Ig antibodies. These data represent one of two repeated experiments. Patients with a P/N ratio of <0.74 were statistically found to be “PTP defective.”