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FA administration impairs colony formation capacity and motivates apoptosis in ESCC cells. (a–c) The colonies were counted when EC-1 and TE-4 cells were administrated with 20 μM, 40 μM, and 60 μM FA for 48 h. (d–f) Apoptotic level of EC-1 and TE-4 cells with 48 h administration of 20 μM, 40 μM, and 60 μM FA was tested via Annexin V-FITC apoptosis staining. p was computed through one-way ANOVA test. Significance level was denoted as ∗∗p < 0.01 and ∗∗∗∗p < 0.0001.
