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. 2022 Oct 11;2022:4607966. doi: 10.1155/2022/4607966

Figure 6.

Figure 6

DFO impairs the stimulative effect of FA on lipid peroxidation and ROS generation in ESCC cells. (a, b) MDA content was measured in EC-1 and TE-4 cells with 48 h administration of 40 μM FA and 50 μM DFO by MDA content kit. (c, d) SOD activity was tested in EC-1 and TE-4 cells that were administrated with 40 μM FA and 50 μM DFO for 48 h through adopting SOD activity kit. (e, f) GSH content was assayed in EC-1 and TE-4 cells under exposure to 40 μM FA and 50 μM DFO for 48 h. (g–i) Intracellular ROS accumulation was assessed in EC-1 and TE-4 cells with 48 h administration of 40 μM FA and 50 μM DFO via DCFH-DA probe. Scale bar, 20 μm. p was computed through one-way ANOVA test. Significance level was denoted as ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.