FIG. 2.

Selective recognition of defined Vsp products of M. bovis PG45 by serum antibodies from M. bovis-infected cattle. Identical Western blots representing the total proteins of three clonal variants (lanes 1 to 3) derived from M. bovis PG45 were respectively immunostained with MAb 1E5 (A), with sera collected from experimental infection (B to F), or with sera collected from natural outbreaks (G to K). The sera used in this experiment are described in Tables 1 and 2 and correspond to animal A009 before (B) and after (C) infection; animal M before (D) and after (E) infection; sera E095 (F), 30 (G), and 56 (H) from two animals of outbreak 4; serum 6241 (I) from outbreak 2; serum 47 (J) from outbreak 1; and serum 283/17 (K) from the field sampling. Lanes 1 through 3 represent clonal variants expressing 79-kDa VspC (lane 1), 64-kDa VspA plus 46-kDa VspB (lane 2), and 67-kDa VspA (lane 3).