Figure 1.

Arginine utilization regulators facilitate spore germination

(A) Spores of PY79 (WT), LR31 (ΔrocR), LR30 (ΔahrC), LR32 (ΔrocR ΔahrC), LR205 (ΔrocR ΔahrC, amyE::rocR-ahrC) strains were induced to germinate on an agarose pad supplemented with L-Ala (10 mM) (t = 0) and followed by time-lapse microscopy. Shown are phase contrast images captured at the indicated time points from a representative experiment out of three independent biological repeats. Scale bar, 1 μm.

(B) Quantification of the experiment described in (A). Data are presented as percentages of the initial number of the phase bright spores. Shown are average values and SD obtained from three independent biological repeats (n ≥ 300 for each strain).

(C) Spores of PY79 (WT), LR31 (ΔrocR), LR30 (ΔahrC), LR32 (ΔrocR ΔahrC), LR205 (ΔrocR ΔahrC, amyE::rocR-ahrC) strains were incubated with L-Ala (10 mM) to trigger germination. DPA release was determined by monitoring the relative fluorescence units (RFU) of Tb³⁺-DPA. Shown is a representative experiment out of three independent biological repeats.