| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| PY79 (B. subtilis wild type) | (Youngman et al., 1984) | NA |
| BKK30700 (rpmEB::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK22880 (ypfD::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK28470 (lysC::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK40350 (rocR::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK05850 (gmuR::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK29010 (speD::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK05950 (ydiF::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK35940 (rbsA::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK25100 (zur::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK15770 (prkC::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK13400 (ykoU::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK19430 (cdaS::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK00650 (yabS::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK13350 (ykoN::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK00630 (yabR::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK33620 (yvaK::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK02330 (ybfQ::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK29830 (ytpQ::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK13250 (ykoG::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK10000 (yhaH::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK05770 (ydhI::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK31390 (yugI::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK24250 (ahrC::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| BKK37790 (rocG::kan) | B. subtilis Strain Collection (Koo et al., 2017) | NA |
| LR14 (mls-turboID-ywlEHAx2) | This study | NA |
| LR30 (ahrC::kan) | This study | NA |
| LR31 (rocR::mls) | This study | NA |
| LR32 (rocR::mls, ahrC::kan) | This study | NA |
| LR33 (gudB::tet) | This study | NA |
| LR38 (gudB::tet, rocR::mls, ahrC::kan) | This study | NA |
| LR127 (gudB::tet, rocR::mls, ahrC::kan amyE::PIPTG-rocG-spc) | This study | NA |
| LR133 (gudB::tet, rocR::mls, ahrC::kan amyE::PspoIID-rocG-cat) | This study | NA |
| LR134 (gudB::tet, rocR::mls, ahrC::kan amyE::PsspE-rocG-cat) | This study | NA |
| LR137 (gudB::tet, rocG::kan) | This study | NA |
| LR197 (rocABC::spc) | This study | NA |
| LR198 (rocDEF::cat) | This study | NA |
| LR200 (gudB::tet, rocABC::spc) | This study | NA |
| LR201 (gudB::tet, rocDEF::cat) | This study | NA |
| LR203 (gudB::tet rocABC::spc, rocDEF::cat) | This study | NA |
| LR205 (rocR::mls, ahrC::kan amyE::rocR-ahrC-cat) | This study | NA |
| LR207 (gudB::tet, rocR::mls, ahrC::kan amyE::rocR-ahrC-cat) | This study | NA |
| BDP87 (amyE::PspoIIQ-lucH245F-cat) | A gift from Prof. Lee Kroos (Parrell and Kroos, 2020). | NA |
| LR209 (gudB::tet amyE::PspoIIQ-lucH245F-cat) | This study | NA |
| LR211 (gudB::tet, rocR::mls, ahrC::kan amyE::PspoIIQ-lucH245F-cat) | This study | NA |
| LR225 (sacA::PIPTG-rocG-spc) | This study | NA |
| LR227 (gudB::tet, rocR::mls, ahrC::kan amyE::PspoIIQ-lucH245F-cat sacA::PIPTG-rocG-spc) | This study | NA |
| Chemicals, peptides, and recombinant proteins | ||
| Chloramphenicol | Sigma-Aldrich | Cat#: C0378 |
| Tetracycline | Sigma-Aldrich | Cat#: 87128 |
| Kanamycin | US Biological | Cat#: K0010 |
| Lincomycin | Sigma-Aldrich | Cat#: 62143-5G |
| Erythromycin | Sigma-Aldrich | Cat#: E0774 |
| Spectinomycin | Sigma-Aldrich | Cat#: S4014-5G |
| Ampicillin | Sigma-Aldrich | Cat#: A9518-25G |
| L-Alainine | MP BIOMEDICALS | Cat#: 100287 |
| Difco™ Nutrient Broth | BD Biosciences | Cat#: 234000 |
| L-aspargine | Sigma-Aldrich | Cat#:A8381-100G |
| D-glucose | Sigma-Aldrich | Cat#:1632-1KG |
| D-fructose | Sigma-Aldrich | Cat#:F3510-500G |
| Histodenz | Sigma-Aldrich | Cat#:D2158-100G |
| RQ1 DNase | Promega | Cat#:M610A |
| Terbium(III) chloride hexahydrate | Sigma-Aldrich | Cat#:212903 |
| Critical commercial assays | ||
| Q5 High-Fidelity DNA Polymerase | NEW ENGLAND BioLabs | Cat#: M0491S |
| Gibson Assembly Master Mix | NEW ENGLAND BioLabs | Cat#: E2611L |
| Quick Ligation Kit | NEW ENGLAND BioLabs | Cat#: M2200S |
| ENLITEN® ATP Assay System | Promega | Cat#:FF2000 |
| Halt™ Protease Inhibitor Cocktail (100X) | Thermo | Cat#: 78438 |
| iScript™ cDNA Synthesis Kit | BIO-RAD | Cat#:170-8891 |
| iTaq Universal SYBR Green Supermix | BIO-RAD | Cat#: 1725124 |
| FastRNA PRO™ BLUE KIT | MP BIOMEDICALS | Cat#: 116025050 |
| Recombinant DNA | ||
| pDR111 (amyE::PIPTG-spc) | a gift from David Rudner (Harvard Medical School) | NA |
| pDG364 (amyE::cat) | (Guerout-Fleury et al., 1996) | NA |
| pET2 (amyE::gfp-cat) | laboratory stock | NA |
| pLR4 (amyE::PIPTG-rocG-spc) | This study | NA |
| pLR5 (amyE::PspoIID-rocG-cat) | This study | NA |
| pLR6 (amyE::PsspE-rocG-cat) | This study | NA |
| pLR7 (amyE::ProcR-rocR-PahrC-ahrC-cat) | This study | NA |
| Sequence-based reagents | ||
| Primers used in this study are listed in Table S1. | All primers were designed during this study, and synthesized by Integrated DNA Technologies (IDT). | NA |
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