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. 2022 Oct 13;8(10):1191–1201. doi: 10.1038/s41477-022-01253-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Flowchart of the data processing for the PSI dimer and monomer. The processing steps performed in cryoSPARC and RELION are indicated. To increase the numbers of Pc bound particles Dataset 2 was added for focused classification with signal subtraction. b, The map of the dimer and symmetry expanded dimer colored by local resolution in two orientations (left). Angular distribution of the dimer and symmetry expanded dimer and the FSC-curves are shown. c, The map of the monomer, angular distribution and FSC-curve. d, The map with the best density for plastocyanin, angular distribution and FSC-curve.