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. 2022 Oct 18;12:16952. doi: 10.1038/s41598-022-20970-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Efficient HIV-1 infection in non-dividing bovine cells. MDBK TRIM5 k/o cells (clones #4–6) cultured in the presence of DMSO or 1 µg/mL Aphidicolin (Aph) were infected with an HIV-1 or MLV vector encoding ZsGreen reporter protein. The infectivity was measured using a flow cytometer 2 days after infection. The results are presented as the mean and standard deviation of hexaplicate measurements from one assay, and they are representative of at least three independent experiments. Differences were examined by a two-tailed, unpaired Student’s t-test. ****p < 0.0001, ns (not significant).