Table 1.

Phospholipidation enables adsorption of UM-3003 to alum and Infanrix vaccine.

Sample (Alum: UM-3003 Salt)	Time point	Time (min)	Peak area	% Adsorbed
1:2 Alum: UM-3003 (aq)	0 (< 5 min)	12	19.81	96.5
	15 min	18	8.41	98.5
	30 min	34	0.06	100.0
	60 min	68	n.a	100.0
	23 h	1380	n.a	100.0
1:1 Alum: UM-3003 (aq)	0 (< 5 min)	12	12.04	97.8
	15 min	18	n.a	100.0
	30 min	34	n.a	100.0
	60 min	68	n.a	100.0
	23 h	1380	n.a	100.0
Alum control	0 (< 5 min)		n.a	0.0
	23 h		n.a	0.0
UM-3003 (aq) control	0 (< 5 min)		160.1
	23 h		175.11
1:4 Infanrix Alum: UM-3003 (aq)		1		99.1
		5		99.4
		7		99.9
		10		99.9
		15		100.0
		30		100.0
		76		100.0
		180		99.9
		1260		99.9

Adsorption kinetics of UM-3003 [molecular weight (MW) of 1047.42)] onto 2% alhydrogel alum and DTaP alum respectively. Aluminum hydroxide derived from DTaP (Infanrix) vaccine, which has preexisting pertussis antigen adsorbed to its surface, was evaluated up to 23 h. UM-3003 fully adsorbed (~ 96–100%) to the alum/antigen within 30 min, with or without excess alum.