Fig. 2. Functional screening of a chloroquine-resistance gene from the genomic libraries of strain Dd2.
a Parasites [dd2-lib1 (red), 2 (blue), 3D7 (black), Dd2 (orange), and the negative control parasite (yellow)] were treated with 20-nM chloroquine for 6 days. The transgenic parasite, in which the pFCENv1 was introduced, was used as a negative control. b The IC50 values of clonal parasites selected from dd2-lib1 and 2 were determined from The values for strains 3D7 and Dd2 and the negative control parasite was also determined. All assays were performed using n = 4 biological independent samples. Error bars are SEM. The color specification is the same as that used in a. c Genomic DNA fragments were obtained from surviving parasites in dd2-lib1 and 2. DNA fragments overlapped at the genomic region encoding the pfcrt gene. Source data are provided as a Source Data file.