Fig. 3. Functional screening of a mefloquine-resistance gene from the genomic libraries of strain MEF1.

a Parasites in mef-lib3 (red), and 6 (blue), 3D7 (black), MEF1 (orange), and the negative control parasite (yellow) were treated for 6 days with 15-nM mefloquine. b Four genomic DNA fragments were recovered from the parasites, which were selected from mef-lib3, 6, 10, and 13. c The IC₅₀ values of clonal parasites selected from mef-lib3, mef-lib-6, 3D7, MEF1, and the negative control parasite were determined. All assays were performed using n = 4 biological independent samples. Error bars are SEM. The color specification is the same as that used in a. Source data are provided as a Source Data file.