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. 2022 Oct 18;13:6172. doi: 10.1038/s41467-022-33697-9

Table 2.

Quantities obtained either by calculations based on formulae shown in Table 1 or by fitting the datasets specified

Quantity Input valuesa/conditions Formulae/dataset Output value
ΔΔGpart. per FG repeat increase

CNaCl = 0.15 mol/dm3,

T = 294 K

Partition coefficient measurements

(Fig. 1d, e)

−255 J/mol
ΔΔGpart. per FG repeat per 1 M increase in ionic strength T = 294 K −282 J/mol
ΔG for phase separation

N = 52,

CNaCl = 0.15 mol/dm3,

T = 300 K

Formula Set 1 −17,600 J/mol
Formula Set 2 −16,500 J/mol
Saturation concentration Formula Set 1 3.89 × 10−6 mol/dm3
Formula Set 2 6.12 × 10−6 mol/dm3
ΔΔG per 1 K increase in temperature

N = 52,

CNaCl = 0.15 mol/dm3

Formula Set 1 −494 J/mol
Formula Set 2 −375 J/mol
ΔΔG per 1 M increase in ionic strength

N = 52,

T = 300 K

Formula Set 1 −19,300 J/mol
Centrifugation dataset (Fig. 3e) −15,200 J/mol
ΔΔG per FG repeat increase

CNaCl = 0.15 mol/dm3,

T = 294 K

Formula Set 2 −202 J/mol
ΔΔG per FG repeat per 1 K increase in temperature CNaCl = 0.15 mol/dm3 Formula Set 2 −5.66 J/mol
ΔΔG per FG repeat per 1 M increase in ionic strength T = 300 K Centrifugation dataset (Fig. 7b) −403 J/mol

aInput values for calculations are selected such that outputs from orthogonal formulae/datasets (DLS, centrifugation and partition datasets) can be compared. Note the high consistency of numbers derived from orthogonal methods.