Figure 11.

scRNA-seq analysis of tuft cells in IPMK^f/fand IPMK^ΔIECmice. (A) An experimental scheme of fluorescence-activated cell sorting (FACS) sorting and plate-based scRNA-seq analysis. (B) A t-distributed stochastic neighbor embedding (t-SNE) plot showing that 2 independent experimental batches were overlaid well, indicating no batch effect. For each experimental batch, 2 IPMK^f/f mice and 2 IPMK^ΔIEC mice were used. (C) Primary unsupervised hierarchical clustering analysis showed 2 clusters of cells. (D) t-SNE plots showing the expression patterns of epithelial cell marker genes (Krt8 and Cdh1), confirming the epithelial identity of analyzed cells. (E) t-SNE plots showing the expression patterns of tuft cell marker genes (Dclk1, Siglecf, and Il17rb). (F) t-SNE plots showing the expression patterns of non–tuft cell marker genes (Atoh1, secretory cells; Krt19, isthmus; and Lypd8, colonic enterocyte).