Figure 3.
IPMKΔIECmice appear normal in homeostatic conditions. (A) IPMK was specifically depleted in intestinal epithelium using Villin-Cre mice. loxP sequences were inserted in the exon 6 region of the Ipmk gene, and Cre-driven deletion of flanked sequences resulted in truncated mRNA transcripts that were degraded readily. Tissue-specific IPMK knockout was confirmed at the (B) mRNA level and (C) protein level (n = 5, 3 per genotype in panel B). Red arrows indicate the IPMK band. Data are presented as means ± SD. (D) Body weights of 10-week-old mice showed no difference between genotypes (n = 6, 3 per genotype). Data are presented as means ± SD. (E) Gut permeability did not differ significantly between genotype in a normal state. Serum FITC-dextran concentration was measured 4 hours after a 400-mg/kg dose by oral gavage (n = 6, 6 per genotype). Data are presented as means ± SD. (F) No notable difference was observed in blots of mTOR complex and AKT signaling cascade. ∗P < .05, ∗∗P < .01. HSP90, heat shock protein 90; UTR, untranslated region.