Figure 7.
Transcriptomic analysis of colonic epithelial cells at an earlier colitis phase and suggested tuft cell–related genes as major remarks of more severe colitis shown in IPMKΔIECmice. (A) The top 5 gene ontology terms were calculated using adjusted P values. (B) Enrichment set data showed significant changes of collagen fibril organization and external encapsulating structure organization by gene set enrichment analysis. (C) Heatmap representing the overlapping DEGs between colonic tissue and colonic epithelial cell mRNA sequencing. Among very few genes, tuft cell marker genes were listed as common DEGs. Mice were killed on day 8 for RNA sequencing of colonic epithelial cells (n = 3, 3 per genotype). FDR, false discovery rate.