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. 2022 Sep 28;37:100455. doi: 10.1016/j.jbo.2022.100455

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — The abundance of circCYP51A1 was up-regulated in OS cells under hypoxia. (A) The expression of circCYP51A1 was determined by qRT-PCR in OS tissues (n = 33) and neighboring normal samples (n = 33). (B) CircCYP51A1 expression in OS cell lines (U2OS and MG63) and human osteoblast cell line (hFOB1.19) was examined by qRT-PCR. (C-D) The expression level of circCYP51A1 was determined in U2OS and MG63 cells with or without RNase R treatment. (E-F) Relative expression of circCYP51A1 in U2OS and MG63 cells exposed to hypoxia treatment for 0, 3, 6, 12, 24, or 48 h was detected by qRT-PCR. *P < 0.05.