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. 2022 Sep 28;37:100455. doi: 10.1016/j.jbo.2022.100455

Fig. 3.

Fig. 3

CircCYP51A1 acted as a sponge of miR-490-3p in OS cells. (A) The binding sites between miR-490-3p and circCYP51A1 were predicted by starbase. (B) The overexpression efficiency of miR-490-3p was detected by qRT-PCR in U2OS and MG63 cells. (C-D) The binding relationship between circCYP51A1 and miR-490-3p was verified by dual-luciferase reporter assay in U2OS and MG63 cells. (E-F) The expression of miR-490-3p in OS tissues and cells was tested by qRT-PCR. (G) Correlation analysis of circCYP51A1 and miR-490-3p expression in OS tissues. (H) The knockdown efficiency of miR-490-3p inhibitor was detected by qRT-PCR. (I) miR-490-3p expression was checked by qRT-PCR in U2OS and MG63 cells transfected with si-NC, si-circCYP51A1, si-circCYP51A1 + in-miR-NC, or si-circCYP51A1 + in-miR-490-3p. (J-K) Relative expression of miR-490-3p in U2OS and MG63 cells exposed to hypoxia treatment for 0, 3, 6, 12, 24, or 48 h was detected by qRT-PCR. *P < 0.05.