Fig. 4.

Silencing of circCYP51A1 impaired the proliferation, migration, invasion and glycolysis of OS cells under hypoxia by targeting miR-490-3p. (A-H) U2OS and MG63 cells were transfected with si-NC, si-circCYP51A1, si-circCYP51A1 + in-miR-NC, or si-circCYP51A1 + in-miR-490-3p after treatment of hypoxia. (A) Cell proliferative capacity was evaluated by colony formation assay. (B-D) Cell migration and invasion were measured by using transwell assays or wound healing assay. (E-F) Lactate production and glucose consumption levels in U2OS and MG63 cells were detected using lactate and glucose assay kits, respectively. (G-H) The protein levels of GLUT1, HK2 and LDHA were examined by western blot. *P < 0.05.