Fig. 6.

KLF12 overexpression reversed the effect of miR-490-3p overexpression on OS progression under hypoxia. (A-H) U2OS and MG63 cells were transfected with miR-NC, miR-490-3p, miR-490-3p + pcDNA or miR-490-3p + KLF12 after treatment of hypoxia. (A) Cell proliferative capacity was evaluated by colony formation assay. (B-D) Cell migration and invasion were measured by using transwell assays or wound healing assay. (E-F) Lactate production and glucose consumption levels in U2OS and MG63 cells were detected using lactate and glucose assay kits, respectively. (G-H) The protein levels of GLUT1, HK2 and LDHA levels were examined by western blot. *P < 0.05.