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LC gradient condition
| Column | ACQUITY UPLC BEH C18 column (1.7 μm, 2.1 × 50 mm), ACQUITY UPLC BEH C18 vanguard pre-column (1.7 μm, 2.1 × 5 mm) |
| Column temperature | 45°C |
| Injecting volume | 5.0 μL |
| Flow rate | 0.15 mL/min |
| Time (min) | Gradient B % |
| 0 | 20.0 |
| 1.0 | 20.0 |
| 3.0 | 60.0 |
| 20.0 | 98.0 |
| 20.1 | 20.0 |
| 22.0 | 20.0 |
| 25.0 | 20.0 |
| Diverter time program | |
| Time (min) | Position |
| 0.0 | B |
| 3.0 | A |
| 15.0 | B |
See also Table S1.
