Table 4.
Phospholipid-specific MRM settings
| Compound name | Q1 | Q3 | DP | EP | CE | CXP | Dwell time (ms) | Retention time (min) |
|---|---|---|---|---|---|---|---|---|
| LPC(16:0) | 554.346 | 255.233 | -80 | -10 | -50 | -10 | 1 | 4.1 |
| LPC(16:1) | 552.331 | 253.217 | -80 | -10 | -50 | -10 | 1 | 3.3 |
| LPC(18:1) | 580.362 | 281.249 | -80 | -10 | -50 | -10 | 1 | 4.1 |
| PC(14:0-14:0) | 736.513 | 227.202 | -80 | -10 | -50 | -10 | 1 | 6.1 |
| PC(14:0-16:0) | 764.545 | 227.202 | -80 | -10 | -50 | -10 | 1 | 6.5 |
| PC(14:0-18:0) | 792.576 | 227.202 | -80 | -10 | -50 | -10 | 1 | 7.1 |
| PC(16:0-16:0) | 792.576 | 255.233 | -80 | -10 | -50 | -10 | 1 | 7.0 |
| PC(16:0-18:0) | 820.607 | 255.233 | -80 | -10 | -50 | -10 | 1 | 7.2 |
| PC(14:0-14:1) | 734.498 | 225.186 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PC(14:0-16:1) | 762.529 | 227.202 | -80 | -10 | -50 | -10 | 1 | 6.1 |
| PC(14:0-18:1) | 790.56 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.6 |
| PC(14:1-16:0) | 762.529 | 255.233 | -80 | -10 | -50 | -10 | 1 | 6.1 |
| PC(14:1-16:1) | 760.514 | 225.186 | -80 | -10 | -50 | -10 | 1 | 5.5 |
| PC(14:1-18:1) | 788.545 | 281.249 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PC(16:0-16:1) | 790.56 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.5 |
| PC(16:0-18:1) | 818.592 | 281.249 | -80 | -10 | -50 | -10 | 1 | 7.1 |
| PC(16:1-18:0) | 818.592 | 253.217 | -80 | -10 | -50 | -10 | 1 | 7.1 |
| PC(16:1-16:1) | 788.545 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.2 |
| PC(16:1-18:1) | 816.576 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.7 |
| PC(18:0-18:1) | 846.623 | 281.249 | -80 | -10 | -50 | -10 | 1 | 7.8 |
| PC(18:1-18:1) | 844.607 | 281.249 | -80 | -10 | -50 | -10 | 1 | 7.2 |
| LPE(16:1) | 450.263 | 253.217 | -80 | -10 | -50 | -10 | 1 | 3.3 |
| LPE(18:1) | 478.293 | 281.249 | -80 | -10 | -50 | -10 | 1 | 4.2 |
| PE(14:0/16:1) | 660.461 | 253.217 | -80 | -10 | -43 | -10 | 1 | 6.2 |
| PE(14:0/18:1) | 688.492 | 281.249 | -80 | -10 | -43 | -10 | 1 | 6.6 |
| PE(14:1/18:0) | 688.492 | 283.264 | -80 | -10 | -43 | -10 | 1 | 5.0 |
| PE(14:1/16:1) | 658.446 | 253.217 | -80 | -10 | -43 | -10 | 1 | 5.8 |
| PE(14:1/18:1) | 686.476 | 281.249 | -80 | -10 | -43 | -10 | 1 | 6.1 |
| PE(16:0/16:0) | 690.508 | 255.233 | -80 | -10 | -50 | -10 | 1 | 6.5 |
| PE(16:0/16:1) | 688.492 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.6 |
| PE(16:0/18:1) | 716.524 | 281.249 | -80 | -10 | -50 | -10 | 1 | 7.2 |
| PE(16:1/18:0) | 716.524 | 283.264 | -80 | -10 | -50 | -10 | 1 | 6.7 |
| PE(16:1/16:1) | 686.476 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.1 |
| PE(16:1/18:1) | 714.508 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.6 |
| PE(18:0/18:1) | 744.555 | 281.249 | -80 | -10 | -50 | -10 | 1 | 7.2 |
| PE(18:1/18:1) | 742.539 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.6 |
| PE(O-16:1/16:0) | 674.513 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.3 |
| PE(O-16:0/18:1) | 702.544 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.9 |
| PE(O-18:0/16:1) | 702.544 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.3 |
| PE(O-16:1/16:1) | 672.498 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PE(O-18:1/16:1) | 700.529 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.3 |
| PE(O-18:1/18:1) | 728.561 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.8 |
| PE(P-14:1/16:0) | 644.5 | 255.233 | -80 | -10 | -50 | -10 | 1 | 4.3 |
| PE(P-16:0/16:1) | 672.5 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.0 |
| PE(P-16:0/18:1) | 700.5 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.5 |
| PE(P-18:0/16:1) | 700.5 | 253.217 | -80 | -10 | -50 | -10 | 1 | 6.5 |
| PE(P-18:0/18:1) | 728.6 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.9 |
| PI(14:0/14:0) | 753.456 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.2 |
| PI(16:0/14:0) | 781.487 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.5 |
| PI(14:0/18:0) | 809.518 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.8 |
| PI(16:0/16:0) | 809.518 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.8 |
| PI(16:0/18:0) | 837.55 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PI(18:0/18:0) | 865.581 | 283.264 | -80 | -10 | -50 | -10 | 1 | 6.3 |
| PI(14:0/14:1) | 751.441 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.0 |
| PI(14:0/16:1) | 779.472 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.2 |
| PI(14:0/18:1) | 807.503 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.5 |
| PI(16:0/14:1) | 779.472 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.3 |
| PI(18:0/14:1) | 807.503 | 283.264 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PI(16:1/14:1) | 777.456 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.0 |
| PI(16:0/16:1) | 807.503 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.5 |
| PI(16:0/18:1) | 835.534 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PI(18:0/16:1) | 835.534 | 283.264 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PI(16:1/16:1) | 805.487 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.3 |
| PI(16:1/18:1) | 833.518 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PI(18:0/18:1) | 863.565 | 283.264 | -80 | -10 | -50 | -10 | 1 | 6.3 |
| PI(18:1/18:1) | 861.55 | 281.249 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PS(14:0/16:1) | 704.451 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.3 |
| PS(14:0/18:1) | 732.482 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PS(14:1/18:1) | 730.476 | 225.186 | -80 | -10 | -50 | -10 | 1 | 5.2 |
| PS(16:0/16:0) | 734.498 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PS(16:0/18:0) | 762.529 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PS(16:1/14:1) | 702.436 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.1 |
| PS(16:0/16:1) | 732.482 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PS(16:0/18:1) | 760.513 | 255.233 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PS(16:1/18:0) | 760.513 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.7 |
| PS(16:1/16:1) | 730.476 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.3 |
| PS(16:1/18:1) | 758.498 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PS(18:1/18:0) | 788.545 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.1 |
| PS(18:1/18:1) | 786.529 | 281.249 | -80 | -10 | -50 | -10 | 1 | 5.7 |
| PG(16:0/16:1) | 719.487 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| PG(16:0/18:1) | 747.518 | 281.249 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PA(14:0/14:0) | 591.403 | 227.202 | -80 | -10 | -50 | -10 | 1 | 5.3 |
| PA(16:1/18:0) | 673.481 | 253.217 | -80 | -10 | -50 | -10 | 1 | 5.9 |
| PA(18:1/18:0) | 701.513 | 281.249 | -80 | -10 | -50 | -10 | 1 | 6.5 |
| PE(17:0/17:0) | 718.57 | 269.264 | -80 | -10 | -50 | -10 | 1 | 7.9 |
| PC(17:0-17:0) | 820.6 | 269.2 | -80 | -10 | -50 | -10 | 1 | 7.8 |
| PS(14:0/14:0) | 678.6 | 227.1 | -80 | -10 | -50 | -10 | 1 | 5.3 |
| PI(17:0/14:1) | 793.49 | 269.25 | -80 | -10 | -50 | -10 | 1 | 5.4 |
| PC(15:0-18:1(d7)) | 811.592 | 288.249 | -80 | -10 | -50 | -10 | 1 | 6.3 |
| PE(15:0/18:1(d7)) | 709.524 | 288.249 | -80 | -10 | -50 | -10 | 1 | 5.7 |
| PS(18:1(d7)/15:0) | 753.531 | 288.249 | -80 | -10 | -50 | -10 | 1 | 6.9 |
| PG(15:0/18:1(d7)) | 740.518 | 288.249 | -80 | -10 | -50 | -10 | 1 | 7.0 |
| PI(18:1(d7)/15:0) | 828.534 | 288.249 | -80 | -10 | -50 | -10 | 1 | 5.7 |
| PA(18:1(d7)/15:0) | 666.481 | 288.249 | -80 | -10 | -50 | -10 | 1 | 5.6 |
| LPC18:1(d7) | 587.362 | 288.249 | -80 | -10 | -50 | -10 | 1 | 4.2 |
| LPE18:1(d7) | 485.293 | 288.249 | -80 | -10 | -50 | -10 | 1 | 4.3 |
Note: When the location of fatty acid chains in a phospholipid is known, the fatty acids are separated by “sn-1/sn-2”. For an unknown location, the fatty acids are separated by a “-” (Buré et al., 2013). The alkyl and alkenyl ether phospholipids are denoted by the “O-” and “P-” prefixes.
See also Table S2.