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Spike-in phospholipid mixture preparation
| Reagent | Final concentration | Amount |
|---|---|---|
| 5 mg/mL 17:0/17:0 PC (chloroform solution) | 420 μg/mL | 84 μL |
| 5 mg/mL 17:0/17:0 PE (chloroform solution) | 420 μg/mL | 84 μL |
| 5 mg/mL 14:0/14:0 PS (chloroform solution) | 420 μg/mL | 84 μL |
| chloroform | 100% (v/v) | 748 μL |
| Total | N/A | 1 mL |
