REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
D-glucose	Sangon Biotech	Cat#A100188-0005; CAS: 50-99-7
Yeast nitrogen base	BD Difco	Cat#11743014
Ammonium sulfate	Sangon Biotech	Cat#A100191-0500; CAS: 7783-20-2
Acetonitrile (LC-MS grade)	Merck	Cat#1590142500; CAS: 75-05-8
Methanol (LC-MS grade)	Merck	Cat#1.06035.2500; CAS: 67-56-1
2-Propanol (LC-MS grade)	Merck	Cat#1.02781.2500; CAS: 67-63-0
Water (LC-MS grade)	Merck	Cat#1.15333.2500; CAS: 7732-18-5
Ammonium acetate (LC-MS grade)	Sigma-Aldrich	Cat#73594-100G-F; CAS: 631-61-8
Chloroform	Sinopharm Chemical Reagent Co., Ltd.	Cat#151902104; CAS: 67-66-3
Citric acid	Sangon Biotech	Cat#A610055-0500; CAS: 77-92-9
17:0 PC (1,2-diheptadecanoyl-sn-glycero-3-phosphocholine)	Avanti	Cat#850360C-25MG; CAS: 70897-27-7
17:0 PE (1,2-diheptadecanoyl-sn-glycero-3-phosphoethanolamine)	Avanti	Cat#830756P-25MG; CAS: 140219-78-9
14:0 PS (1,2-dimyristoyl-sn-glycero-3-phospho-L-serine sodium salt)	Avanti	Cat#840033P-25MG; CAS: 105405-50-3
LIPIDOMIX® Standards (methanol solution)	Avanti	Cat#330707-1EA
Experimental models: Organisms/strains
S. cerevisiae: CEN.PK: MAT a	(van Dijken et al., 2000)	N/A
S. cerevisiae: CEN.PK: MAT a; cho2Δ:: KanMX	This paper	N/A
S. cerevisiae: CEN.PK: MAT a; opi3Δ:: KanMX	This paper	N/A
S. cerevisiae: CEN.PK: MAT a; psd1Δ:: HYGMX	This paper	N/A
Software and algorithms
Analyst v1.7.2	SCIEX	https://sciex.com/products/software/analyst-software
OS v1.7	SCIEX	https://sciex.com/products/software/sciex-os-software
Other
Triple Quad 6500+ QTRAP mass spectrometer	SCIEX	N/A
ACQUITY UPLC BEH C18 column (1.7 μm, 2.1 × 50 mm)	Waters	186002350
ACQUITY UPLC BEH C18 vanguard pre-column (1.7 μm 2.1 × 5 mm)	Waters	186003975
0.55 mm Glass beads	Sigma-Aldrich	Z250465
15 mL Glass tubes	N/A	N/A
5 mL Glass pipettes	N/A	N/A
2 mL clear screw-top sample vials with patch	HAMAG	HM-0713
250 μL glass insert	HAMAG	HM-2085
Sterile syringe filter, 0.22 μm (PES), 33 mm	Merck Millipore	Cat#SLGP033RB-1
Acid-Resistant CentriVap Centrifugal Vacuum Concentrator	Labconco	Cat#7810033
Dual-position snap cap	Crystalgen	23-2059
Screw-cap tubes	NEST	634101-B
26G needle	KDL	N/A
Bead-beater (Automatic sample fast grinder-24 L)	Shanghai Jingxin	JXFSTPRP-24
1.5 mL microcentrifuge tube	VWR	10025-724
15 mL centrifuge tube	VWR	10025-686