Figure 2.
Forced GPX2 expression promotes KRASG12C-driven lung tumorigenesis. (A) BEAS-2B cells were transduced with KRASG12C mutant, GPX2 expression lentivirus or EV control, and then lysates were collected for western blotting. (B) BEAS-2B cells (2,500/well) transduced with indicated vectors were seeded in 96-well plates, and then cell viability assays were conducted at days 0, 2, 4 and 6. (C and D) BEAS-2B cells (10,000/well) transduced with indicated vectors were seeded in 6-well plates for soft agar assays. (C) Representative images and (D) average number of colonies per well were shown. Scale bar, 500 µm. (E and F) BEAS-2B cells transduced with indicated vectors were used for Transwell migration and invasion assays. (E) Representative images and (F) relative migration or invasion cells are shown. Scale bar, 50 µm. (G-I) BEAS-2B cells (2×106) transduced with indicated vectors were subcutaneously injected into nude mice, then tumor xenografts were allowed to grow for 4 weeks. (G) Tumor growth curves, (H) representative images and (I) tumor weight are presented. (J and K) ROS levels were evaluated by flow cytometry. (J) Oxidative DCF-positive cells and (K) relative FACS value are shown. (L) NADPH/NADP+ ratio of BEAS-2B cells transduced with the indicated vectors are presented. *P<0.05. GPX2, glutathione peroxidase 2; KRAS, Kirsten rat sarcoma viral oncogene homolog; EV, empty vector; ROS, reactive oxygen species; DCF, dichlorofluorescein.