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. 2022 Oct 13;16:100476. doi: 10.1016/j.fochx.2022.100476

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — HPLC patterns of purified-anthocyanin elution with different solvents and elution gradients. Mobile phase A: 2 % or 5 % formic acid and mobile phase B: Acetonitrile (A); Mobile phase A: 5 % formic acid and mobile phase B: 5 % formic acid acetonitrile (v/v) (B); Mobile phase A: 5 % formic acid and mobile phase B: methanol acetonitrile (6:4, v/v) (C, D, E). The elution conditions for mobile phase B were: 0–15 min, 5–10 %; 15–45 min, 10–40 %; 45–50 min, 40–90 % (A, B). 0–35 min, 18–40 % (C). 0–20 min, 15–22 %; 20–50 min, 22–40 % (D). 0–30 min, 10–25 %; 30–40 min, 25–35 %; 40–45 min, 35–90 %; 45–50 min, 90 % (E). Column temperature was 30 °C.