Figure S3. Changes in the metabolic conditions increase the relevance of Ubc8.

(A) Volcano plots showing the effect of lactate-to-lactate medium replacement. In comparison with the lactate-to-glucose replacement, the changes were only very moderate. In Δubc8 cells, some mitochondrial proteins were depleted after the addition of a fresh medium. (B) The intensities (z-scores) of all proteins of group 4b at time point t₀ were plotted, indicating that proteins of this group were present at lower levels in the Δubc8 strain compared with those in the wild-type strain. (C) Protein intensities of mitochondrial representatives that are found in group 4b. Glucose addition consistently reduced the levels of these proteins. Please note that the absence of Ubc8 increased the reduction of all these proteins.