Figure S5. Ubc8 increases the levels of Tom22 and is particularly relevant for the mitochondrial accumulation of proteins of the matrix.

(A) Scheme of the proteome analysis. (B) Volcano plot comparing protein levels of wild-type and Δubc8 cells in glycerol-grown cultures before glucose was added. Under these conditions, gluconeogenic enzymes are not degraded, and their level is not diminished in the presence of Ubc8. Positions of proteins of the mitochondrial import machinery (Tom70, Tom71, Tom40, Tom22, Tom20, Tom6, Tom5, Mdm10, Sam50, Sam37, Sam35, Tim50, Tim23, Tim17, Tim44, Pam16, Pam18, Ssc1, Mge1, Tim21, Mgr2, Pam17, Tim22, Tim54, Sdh3, Tim8, Tim9, Tim10, Tim12, Tim13, Mas1, Mas2, Mia40, and Erv1) are indicated. (C, D) WT and Δubc8 cells were grown in a glycerol medium. Cells were harvested, and cell lysates were analyzed by Western blotting using antibodies against Tom22 and Act1 for control. Panel (D) shows mean values and standard deviations of three biological replicates (n = 3). Significance testing was performed with a t test (*P ≤ 0.05). (E) Violin plots show the relative abundance of proteins of the different mitochondrial subcompartments in Δubc8 cells relative to that in wild-type cells. Indicated is the proportion of proteins in the respective mitochondrial subcompartment that are of reduced abundance in Δubc8 cells.

Source data are available for this figure.