Fig. 2.

NUPR1 enhances the radiation resistance of HCC cells in vivo. a, b Subcutaneous tumor formation in nude mice was established with NUPR1-overexpressing or control MHCC-97H cells (n = 5/group). Tumor sizes were measured every 4 days using calipers (left, representative tumor samples; middle, growth curves of subcutaneous tumors; right, the statistical graph of tumor volumes). c Representative images of each group were photographed at the end of the experiment. d Growth curves of subcutaneous tumors (left) and tumor growth rates of mice treated with IR, ZZW-115, or IR plus ZZW-115 were represented (right). e IHC images of Ki67 and γH2AX expression in xenograft tumors derived from MHCC-97H cells with NUPR1 overexpression or empty vector were represented (left, scale bar: 50 μm). The positive stain (in percentages) was analyzed (right). f IHC images of PCNA and γH2AX expression in xenograft tumors derived from Hepa1-6 cells with different treatments were shown (left, scale bar: 50 μm). The positive stain (in percentages) was analyzed (right). Data are the mean of biological triplicates and are shown as the mean ± SD. P values: *P < 0.05; **P < 0.01; ***P < 0.001 by two-tailed Student’s t-test, or by two-way ANOVA