TABLE 1.
Media used to isolate Vibrio cholerae from frozen, stored rice-water stool and vomitus from cholera patientsa
| VOMITUS |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No preservative |
Cultured from + glycerol |
|||||||||||||
| Patient | LB | TSAb | TCBS | LB > LB | APW > LB | LB > TCBS | APW > TCBS | LB | TSAb | TCBS | LB > LB | APW > LB | LB > TCBS | APW > TCBS |
| 1 | X | X | − | X | X | X | X | ND | ND | ND | ND | ND | ND | ND |
| 2 | − | − | − | − | − | − | − | − | − | − | − | X | − | X |
| 3 | − | − | − | − | − | − | − | − | − | − | − | − | − | X |
| 4 | − | − | − | − | − | − | − | X | X | − | − | X | − | X |
| 5 | − | − | − | − | − | − | − | X | X | − | − | X | − | X |
| 6 | − | X | − | X | X | − | − | ND | ND | ND | ND | ND | ND | ND |
| 7 | − | − | − | − | − | − | X | − | − | − | − | − | − | − |
| 8 | − | − | − | − | − | − | − | − | X | − | − | − | − | − |
| 9 | − | − | − | − | − | − | − | X | X | − | − | X | − | X |
| 10 | − | − | − | − | − | − | − | X | X | − | − | X | − | X |
| 11 | − | − | − | X | − | X | − | ND | ND | ND | ND | ND | ND | ND |
| 12 | − | − | − | X | X | − | X | − | − | − | − | − | − | − |
| STOOL | ||||||||||||||
| 1 | X | X | − | X | X | X | X | ND | ND | ND | ND | ND | ND | ND |
| 2 | X | X | − | X | X | X | X | ND | ND | ND | ND | ND | ND | ND |
| 3 | X | X | − | X | X | − | − | ND | ND | ND | ND | ND | ND | ND |
| 4 | − | − | − | X | X | X | X | ND | ND | ND | ND | ND | ND | ND |
| 5 | − | − | − | X | X | X | X | ND | ND | ND | ND | ND | ND | ND |
| 6 | − | − | − | X | X | − | − | ND | ND | ND | ND | ND | ND | ND |
| 7 | − | − | − | X | − | − | X | ND | ND | ND | ND | ND | ND | ND |
| 8 | − | − | − | − | − | − | − | X | X | − | X | X | − | X |
| 9 | − | − | − | − | − | − | − | X | X | − | − | X | − | − |
| 10 | − | − | − | − | − | − | − | X | X | − | − | − | − | − |
| 11 | − | − | − | − | − | − | − | X | − | − | − | − | − | − |
| 12 | − | − | − | − | − | − | − | − | X | − | X | − | X | − |
| 13 | − | − | − | − | − | − | − | − | − | − | − | − | X | − |
X represents successful isolation (the culture grew V. cholerae), “−” indicates a negative culture (V. cholerae did not grow). “>” denotes enrichment in broth (first set of initials) for 24 h prior to plating on agar (second set of initials). All direct plated samples were incubated at 37°C overnight, and enrichment samples underwent one overnight incubation in enrichment media and another after plating onto agar. Overnight enrichment was chosen because this interval was previously found to be equivalent to 6 to 8 h enrichment for Vc recovery (2). If samples stored without preservative did not yield Vc, glycerol-preserved samples were cultured. APW incubations were conducted in nonshaking culture, and LB incubations were in shaking culture at 225 rpm. Patient samples with no successful Vc isolations (7 stool samples and 8 vomitus samples) are not shown. ND, not done; LB, Luria-Bertani; TSAb, tryptic soy agar with 5% sheep’s blood; TCBS, thiosulphate citrate bile salts agar; APW, alkaline peptone water.