TABLE 2.
Reactivity of antihuman IgG subtype-specific probes with protein A- or protein G-purified human, baboon, and macaque IgGa
| Method | IgG Coat | OD410 with
HRP-labeled probeb
|
|||
|---|---|---|---|---|---|
| Anti-IgG1 | Anti-IgG2 | Anti-IgG3 | Anti-IgG4 | ||
| Protein A | Human | 0.650 (0.033) | 0.494 (0.025) | 0.515 (0.026) | 0.489 (0.024) |
| Baboon | 0.299 (0.015) | 0.241 (0.012) | 0.056 (0.003) | 0.136 (0.007) | |
| Macaque | 0.141 (0.007) | 0.151 (0.008) | 0.022 (0.001) | 0.151 (0.008) | |
| Protein G | Human | 0.556 (0.028) | 0.406 (0.020) | 1.496 (0.075) | 0.716 (0.036) |
| Baboon | 0.228 (0.011) | 0.167 (0.008) | 0.084 (0.004) | 0.163 (0.008) | |
| Macaque | 0.303 (0.015) | 0.339 (0.017) | 0.084 (0.004) | 0.168 (0.008) | |
a
Purified IgG preparations were used to coat the solid phase of ELISA plates and directly probed with a pretitrated amount of HRP-labeled antisubtype-specific reagent.
b
Values represent the means of triplicate determinations at OD410 minus background. Numbers in parentheses represent the standard errors of the means.