TABLE 3.

Reactivity of anti-human IgG subtype-specific probes with protein A and protein G cross-purified human, baboon, and macaque IgG^a

Method	IgG coat	OD410 with HRP-labeled probeb
		Anti-IgG1	Anti-IgG2	Anti-IgG3	Anti-IgG4
Protein G	Human	0.122 (0.006)	0.044 (0.002)	1.066 (0.053)	0.063 (0.003)
	Baboon	0.365 (0.018)	0.171 (0.009)	1.142 (0.057)	0.153 (0.008)
	Macaque	0.015 (0.001)	0.022 (0.001)	0.025 (0.001)	0.019 (0.001)
Protein A	Human	0.536 (0.027)	0.361 (0.018)	0.374 (0.019)	0.590 (0.030)
	Baboon	0.222 (0.011)	0.163 (0.008)	0.071 (0.004)	0.162 (0.008)
	Macaque	0.277 (0.014)	0.346 (0.017)	0.054 (0.003)	0.184 (0.009)

^aThe unbound serum from protein A was passed over and bound to protein G, and the unbound serum from protein G was passed over and bound to protein A. 

^bValues represent the means of triplicate determinations at OD₄₁₀ minus background. Numbers in parentheses represent the standard errors of the means.