Fig. 5. Co-administration of heat-iMVA promotes trafficking of antigen-carrying migratory DC into skin draining LN and activation of resident dendritic cells.

C57/B6J mice were intradermally vaccinated with OVA647 (5 μg) in the presence or absence of heat-iMVA (10⁷ pfu). a, b After 24 h, OVA647 intensities in different dendritic cells populations from dLNs were measured. c Representative dot plots of OVA647 expression in CD11b⁺ dendritic cells. d, e After 24 h, CD86 expressions in different dendritic cells populations from dLNs were measured. f Representative dot plots of CD86 expression in CD8⁺ dendritic cells. Data are represented as mean ± SEM (n = 3-5; ^*P < 0.05, and ^**P < 0.01; Two-tailed Mann-Whitney U test). Data are representative of three independent experiments.