Figure 4:

Effects of pharmacologic targeting of FXI by 14E11 on macrophage recruitment following IVC constriction. Quantification of the percent area of Iba1 in the thrombus on days 3 and 7 post IVC constriction in saline- versus 14E11-treated mice (n=4–5, Day 3: 0.4±0.4 versus 0.6±0.4, P=0.41 and Day 7: 2.4±0.6 versus 1.6±0.4, *P=0.029) (A). Quantification of the percent area of Iba1 immunostaining along the vein wall 3 and 7 days post IVC constriction in saline- versus 14E11-treated mice (n=4–5, Day 3: 0.78±0.8 versus 1.1±0.2, P=0.52 and Day 7: 1.6±0.2 versus 1.1±0.2, **P=0.01) (B). Representative images of Iba1 immunostaining of the thrombus and vein wall at 20x (scale bar=0.5 mm) (C). Data were analyzed using Mann-Whitney test comparison and presented as mean±SD.