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. 2020 Jul 22;2:3. doi: 10.3389/frph.2020.00003

Figure 4.

Figure 4

ME-SFCs from endometriosis patients exhibit enhanced cell migration compared to control ME-SFCs; treatment of control ME-SFCs with TNF promotes cell migration. (A) Increased cell migration by ME-SFCs from endometriosis patients. Healthy control ME-SFCs (CTRL, n = 15) and endometriosis ME-SFCs (ENDO, n = 16) were analyzed for cell migration using the scratch assay method as described in the methods section. Monolayers were photographed at time 0 and after 20 h (see Supplemental Figure 2). Images were imported into Image J and cell migration was determined as % wound or scratch closure. Data are shown for ME-SFCs from the controls (white box) and endometriosis subjects (gray box) using Tukey box and whisker plots (box = interquartile range; horizontal line = median; upper and lower whiskers indicate range without outliers; outliers = •). Significance was determined using unpaired students t-tests with Welch's correction. (B) Increased migration of control ME-SFCs after exposure to TNF. Healthy control ME-SFCs (n = 6) were treated with vehicle and TNF (10 ng/ml), in separate flasks, on days 1 and 3. On day 7 cells were analyzed for cell migration using the scratch assay method as described in A. Data are shown as paired data points (after vehicle treatment and TNF treatment) for each healthy control. Significance was determined using a paired students t-test (B). **P < 0.01; ***P < 0.001.