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. 2022 Feb 1;1:817254. doi: 10.3389/fbinf.2021.817254

FIGURE 3.

FIGURE 3

Localization merging. In the shown example, two fluorophores are present. Both fluorophores are emitting for multiple frames, but are also blinking during this period. The localization merging routine identifies emitters that emit over multiple frames, accounting for possible blinking periods. Merging the individual localizations into one increases accuracy of fluorophore quantification and emitter localization precision.