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. 2022 Feb 1;1:817254. doi: 10.3389/fbinf.2021.817254

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Copyright © 2022 Martens, Turkowyd and Endesfelder.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single-particle tracking computational approaches. Left: Individual emitters have to be linked to create trajectories. Individual emitters have been localized at multiple frames (A), and the nearest neighbour localization in frame n+1 is determined for every localization at frame n, and a linkage is created between these localizations (B,C). This creates trajectories that can be further analysed (D). Right: quantification analysis of trajectory data. See the main text for details on the methods.