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The gene expression profiling workflow. The RNA sequencer produces raw RNA read counts that are aligned on the cell’s genome and processed through the quality control (QC) steps. The raw read counts result from QC are next normalized and analyzed statistically to infer the differential gene expressions (DGEs) or other analyses such as Shannon Entropy, Correlations or PCA. Several bioinformatics analyses can also be performed on the list of DEG for functional inference.
