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. 2021 Oct 20;1:724325. doi: 10.3389/fbinf.2021.724325

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Copyright © 2021 Schodt and Lidke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Frame-connection results for actin microfilament localizations generated by multi-emitter fitting. (A) Gaussian reconstruction image of the LAP-FC frame-connection results. Selected ROIs indicated by numbered yellow boxes in (A) are shown for the frame-connection results using (C) LAP-FC with a maximum pre-clustering frame gap of five frames and a maximum separation of 5 times the localization error; (D) the hypothesis test algorithm with a maximum frame gap of five frames, a maximum separation of 1 pixel, and a level-of-significance of 0.05; (E) the classical algorithm with a maximum frame gap of 1 frame and a maximum separation of 0.2 pixels; and (F) the revised classical algorithm with a maximum frame gap of five frames and a maximum separation of 2 times the localization error. Scale bar = 0.5 μm. Localizations in (B)–(F) are displayed as circles of radius equal to the localization error centered on the estimated position and color-coded to indicate time, with dark blue indicating the start of the experiment and yellow indicating the end of the experiment. The red arrows in (B)–(F) ROI 1 point to a qualitatively interesting set of localizations.