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. 2022 Oct 5;9:976956. doi: 10.3389/fmed.2022.976956

Table 5.

β -giardin (bg) multiple alignment sequence isolates in this study with reference sequences retrieved from GenBank, representing position of intra-genotypic substitutions in sub-assemblages of assemblages A and B.

Isolates/GenBank accession no. Nucleotide position from the start of the gene
Assemblage A 177 204 216 273 285 303 333 357 393 417 432 435 460 468 498 516 534 541 564 567 606 624
AII-A2 AY072723 C A G G C A T G T T G T C T A C G G C G T G
AI-A1 X85958 . . . . . . . . . . . . . . . . . . . . C .
AII-A3 AY072724 . . . . . . . . . . . . T C . . . . . . . .
AII-A4 AY545642 . . . . . . . . . . . . . . . . . A . . C .
AII-A3 IGT2,11-14, 20-22, 25-26, 30, 37, 39-40, 117, 143; IGR81,287 . . . . . . . . . . . . T C . . . . . . . .
AII-A2 IGT8, 19, 29, 31, 41 . . . . . . . . . . . . . . . . . . . . . .
AII-A3 IGT9; IGA340 . . . . . . . . . . . . . C . . . . . . . .
AII-A3 IGT213 . . . . . G . . . . . . . C . . . . . . . .
A IGT93 T . R . . . Y . . Y A C . Y . . R . . S . S
A IGT7H . R . R Y R . Y Y Y A . T C M Y R . T S . .
Assemblage B 210 228 273 303 327 354 357 384 393 432 435 438 450 471 538 541 550 564 594 609 610 636 639 645 648
BIII-B2 AY072726 C A G G C C C C C A C C C C G G G T G C G G G C G
BIV-B3 AY072727 . . A . . . T . . . . . . . . . . . . . . . . . .
BIV-B1 AY072725 T . A . T T T . . . . T . . . . . . . . . . . . A
BIV-B4 AY072728 . G A . . T T . . . . . . . . . . C . T . . . T .
BIV-B5 AY647265 . . A . . T T . . . . . . T . . . . . . . . . . .
BIV-B6 AY647266 T . A . . . T . . . . . . . . . . . . . . . . . .
BIV-B3 IGT1,3,6,10,17,23,24,27,28,35,164; IGR12 . . A . . . T . . . . . . . . . . . . . . . . . .
BIV-B6 IGT4 T . A . . . T . . . . . . . . . . . . . . . . . .
BIV IGT5 T . A . . . T . . . . . . . . . . . . . A . . . .
BIV IGT7,15,16,34 . . A . . T T . . . . . . . . . . . . . . . . . .
BIV IGT18; IGR197 . . R . . . T . . . . . . . . . . . . . . . . . .
BIV IGT32 . . A . . . T . . . . . . . . . . R . . . . . . .
BIV IGT33 . . A . . . T . T . . . . . . . . . . . . . . . .
BIV IGT36, 38; IGR101,386 T . A . . T T . . . . . . . . . . . . . . . . . .
BIV IGT52 . G A . . . T . . . . . . . . . . . . . . . . . .
BIV IGT110; IGA305 T . A . . T T . . . . . . . . . . . A . . . . . .
BIV IGT152 . . R . . . T . . . . . . . T C C . . . . . . . .
BIV IGT165 Y . R R . T K T T . . . . . . . . . . . . . . . .
BIV IGT182 . . A . . T T T T G T . . . . . . . . . . . . . .
BIV IGA458 . . A . . . T . . . . . T . . . . . . . . . . . .
BIV IGR519 . . R . . . T . . . . . . . . . . . . . . A T . .

Accession numbers of the isolates used as sub-assemblage reference isolates are included in bold. Numbers in bold represent nucleotide substitutions from the start of the gene, which differentiate between sub-assemblages introduced by Weilinga and Thompson and Cacciò et al. (15, 17) position and breakdown of intra-genotypic substitutions. Heterogeneous positions are indicated by standard IUPAC codes in bold. Dots indicate nucleotide identity to the AII (AY072723) or BIII (AY072726) reference sequences.