TABLE 2.

Mean number of sequences and percentage of protein-coding genes (CDS genes), and the relative abundance of CDS assigned to taxa at the domain level for the three Hg contamination levels.

	Low Hg	Moderate Hg	High Hg	P +
Raw reads (× 106)	99.2	85.8	82.7	0.34
High-quality reads (× 106)	97.2	84.1	81.1	0.34
Reads aligned to contigs (%)	77.5	72.8	72.1	0.05
Aligned reads mapped to CDS genes (%)	78.6	77.1	77.6	0.24
Bacteria (%)	58.7	58.2	57.6	0.07
Archaea (%)	0.90	0.81	0.89	0.78
Eukarya (%)	0.23	0.23	0.23	0.54
Viruses (%)	0.03	0.03	0.03	0.91
Unclassified (%)	40.1	40.8	41.2	0.04

⁺Effect of Hg contamination assessed by analysis of variance (ANOVA); significant values (P < 0.05) are in bold.