Table 3.
Relative amounts (%, mean + standard deviation) of the most abundant compounds identified in the A. donax leaf cuticular waxes.
| Compound | Chain length | Log | Adaxial | Abaxial | ||
|---|---|---|---|---|---|---|
| Epicuticular | Intracuticular | Epicuticular | Intracuticular | |||
| 1-octacosanol | C28 | 12.6 | 2.7 ± 0.9 | n.d. | 5.4 ± 1.3 | 3.5 ± 1.8 |
| 1-triacontanol | C30 | 13.6 | 12.5 ± 2.7 | 16.2 ± 3.4 | 23.9 ± 9.7 | 15.4 ± 2.5 |
| 1-dotriacontanol | C32 | 14.6 | 3.8 ± 1.0 | 5.6 ± 1.6 | 4.6 ± 1.4 | 3.1 ± 0.7 |
| Total alcohols | 27.5 ± 2.9BC | 30.0 ± 3.7AB | 31.8 ± 5.5A | 24.9 ± 4.8C | ||
| monopalmitin a | C19 | 5.6 | 1.9 ± 0.2 | 1.8 ± 0.1 | n.d. | 1.3 ± 0.0 |
| octadecyl acetate | C20 | 8.7 | 8.0 ± 1.1 | 7.7 ± 0.9 | 2.4 ± 0.6 | 2.6 ± 0.6 |
| eicosyl acetate | C22 | 9.7 | 4.2 ± 0.6 | 4.1 ± 0.4 | n.d. | 1.7 ± 0.1 |
| Total esters | 14.0 ± 1.5A | 13.6 ± 1.2A | 2.4 ± 0.6B | 3.6 ± 1.5B | ||
| stigmasterol | C29 | 9.4 | 5.6 ± 0.9 | 6.8 ± 1.3 | 2.8 ± 1.0 | 3.3 ± 0.6 |
| lupeol | C30 | 9.2 | n.d. | n.d. | 15.9 ± 8.5 | 20.6 ± 1.9 |
| β-amyrin | C30 | 9.2 | 42.3 ± 4.0 | 38.5 ± 3.1 | 25.1 ± 8.7 | 32.2 ± 3.9 |
| Total triterpenoids | 48.7 ± 3.7C | 45.3 ± 3.1C | 57.5 ± 6.1B | 62.7 ± 4.4A | ||
| palmitic acid | C16 | 7.2 | 4.6 ± 1.5 | 4.4 ± 1.2 | 2.3 ± 0.7 | 2.4 ± 0.4 |
| triacontanal | C30 | 13.6 | 5.6 ± 1.8 | 7.0 ± 2.0 | 6.6 ± 1.1 | 7.2 ± 0.8 |
Different letters in a row correspond to statistically significant (p<0.05) differences between means of major chemical classes.
n.d., not detected.
a
Sum of both positional isomers.
b
Predicted data generated using the US Environmental Protection Agency’s EPISuite™.