Fig 4. The Ultramel color morph is associated with a missense variant and a putative deletion in TYRP1.

(A) Alignment of TYRP1 protein sequence surrounding missense variant R305H. (B) Genotypes of 11 Ultramels and 78 Non-Ultramels. This set of animals includes the original Ultramel animal in which the R305H variant was identified. Ultramels heterozygous for R305H are presumed to be heterozygous for the putative deletion of coding regions 6 and 7; testing these animals for the putative deletion was not possible because the putative deletion cannot be detected when heterozygous. (C) Top, schematic of the TYRP1 gene. Bottom left, PCR amplifications demonstrating the putative deletion in the Ultramels lacking R305H. Bottom right, alignment of PCR amplicons to the TYRP1 gene. (D) Schematic of the two TYRP1 haplotypes found in Ultramels. (A, D) Hash mark, discontinuity in the Burmese python reference genome.