Skip to main content
. 2022 Oct 4;13:1020902. doi: 10.3389/fimmu.2022.1020902

Figure 2.

Figure 2

MMP-7 is up-regulated by inflammatory mediators in epithelial cells, smooth muscle cells, and macrophages of the colon. (A) RT-qPCR analysis of MMP7 mRNA levels in human FHC (white bars), rat colonic smooth muscle (RCSMC, black bars), and human THP-1 (gray bars) cells treated with 10 ng/mL indicated cytokine or LPS. RT-qPCR assays also determined the MMP7 mRNA levels in the FHC cells treated with serial doses of IL-1β (B), TNFα (C), or LPS (D), in RCSMC treated with IL-1β (E), IL-4 (F), or IL-13 (G), and in THP-1-derived macrophages treated with IL-1β (H), TNFα (I), or LPS (J) for 24 hours. Time course experiments were performed to evaluate temporal changes of MMP7 mRNA expression in FHC cells treated with 10 ng/ml IL-1β (K) or 100 ng/ml TNFα (L), in RCSMC treated with 10 ng/ml IL-1β (M), 100 ng/ml IL-4 (N), or 100 ng/ml IL-13 (O), and in THP-1 cells treated with 10 ng/ml IL-1β (P), 10 ng/ml TNFα (Q), or 100 ng/ml LPS (R). n=3 or 4. *p<0.01.