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. 2022 Oct 4;13:1020902. doi: 10.3389/fimmu.2022.1020902

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Copyright © 2022 Xiao, Lian, Zhong, Krishnachaitanya, Cong, Dashwood, Savidge, Powell, Liu and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MMP-7 knockout prevents DSS-induced suppression of Claudin-7 in the colon. C57BL/6 and Mmp7 ^-/- mice received 3% DSS in drinking water for 7 days as shown in Figure 4 . Full thickness colon tissue was fixed in formalin for immunohistochemical analysis or snap-frozen in liquid nitrogen for immunoblot analysis. (A) Representative images of immunofluorescence staining for MMP-7 in the colons of C57BL/6 and Mmp7 ^-/- mice treated with or without DSS. (B) Quantification of MMP-7 staining intensity. (C) Representative images of WB for indicated proteins in the colon. (D–F) Protein expression levels determined by densitometry. Arbitrary optical density units of the targeting proteins were normalized vs β-actin and presented as fold change. n=5. *p<0.01 vs WT control. NS, not significant.