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. 2022 Oct 19;17(10):e0275742. doi: 10.1371/journal.pone.0275742

Table 5. Inositol and inositol phosphate (InsP2-6) levels (nmol/g wwt) in kidney of day 21 broilers1,2.

Diet Inositol InsP2 InsP3 InsP4 InsP5 InsP6 Σ InsP
Control 6430±480 1.4±0.2 3.3±1.0 27.0±5.4 40.7±6.0 15.4±2.4 87.9±12.5
Phy500 6600±260 0.9±0.2 1.2±0.1 14.0±2.1 31.5±3.0 10.6±2.1 58.1±5.5
Phy6000 7530±310 0.7±0.2 1.7±0.6 9.8±1.5 15.9±3.1 5.2±0.73 33.4±5.1
Probabilities
Control vs. Phy500 0.770 0.182 0.203 0.203 0.366 0.366 0.203
Control vs. Phy6000 0.134 0.039 0.182 0.023 0.006 0.003 0.003
Phy500 vs. Phy6000 0.130 0.611 0.570 0.317 0.013 0.120 0.019

Abbreviations: Σ InsP, total InsP2 to InsP6; InsP6, inositol hexakisphosphate; InsP5, inositol pentakisphosphate; InsP4, inositol tetrakisphosphate; InsP3, inositol trisphosphate; InsP2, inositol bisphosphate.

1The control group was fed with a diet with 0.45% calculated available phosphate. Groups Phy500 and Phy6000 were fed with the control diet supplemented with 500 or 6,000 FTU of phytase per kilogram of feed, respectively.

2Data are given as group means ± SEM, n = 12, of 6 replicate pens with samples taken from 2 broilers per pen per treatment. Statistical analysis was performed by multiple T tests with correction for multiple comparisons using the Holm-Šídák method for inositol, inositol phosphate and Σ InsP data.