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. 2022 Oct 19;8(42):eadc9022. doi: 10.1126/sciadv.adc9022

Fig. 7. Inhibition of the sulfotransferase Sult1e reverses sterol misbalance and activates LXR.

Fig. 7.

(A) Cartoon illustrating the triclosan injection strategy (50 mg/kg of body weight; intraperitoneal) in 12-week-old Atp7b−/− male mice to inhibit Sult1e1. (B) Liver oxysterols (4-OHC, 7-OHC, 24-OHC, 25-OHC, and 27-OHC) were significantly reduced in KO mice, and Sult1e1 inhibition by triclosan improves 24-OHC and 25-OHC level (green box). (C) Sulfated oxysterols (24-OHCS, 25-OHCS, 27-OHCS, and DHEAS) were significantly elevated in KO mouse liver and reduced by Sult1e1 inhibition. Values represent means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; n = 6 male mice per group. (D) LXR inflammatory targets (iNOS, IL-1β, COX2, IL-6, and TNFα) were significantly elevated in untreated KO mice compared to Het. Treatment with triclosan down-regulates the expression of inflammatory LXR target genes and up-regulates lipogenesis (FASN and SCD1) in KO mice. Values represent means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001; n = 5 to 10 male mice per group. Blue color denotes Het + corn oil, red color denotes KO + corn oil, and magenta color denotes KO + triclosan, respectively.